CHANGES IN THE ACTIVITY AND SUBCELLULAR DISTRIBUTION OF PPO IN ‘DELICIOUS’ APPLES DURING CONTROLLED ATMOSPHERE STORAGE

  1. DIANE M. BARRETT1,*,
  2. C. Y. LEE2,
  3. FRANK W. LIU3

Article first published online: 23 FEB 2007

DOI: 10.1111/j.1745-4514.1991.tb00154.x

Issue

Journal of Food Biochemistry

Journal of Food Biochemistry

Volume 15, Issue 3, pages 185–199, September 1991

Additional Information

How to Cite

BARRETT, D. M., LEE, C. Y. and LIU, F. W. (1991), CHANGES IN THE ACTIVITY AND SUBCELLULAR DISTRIBUTION OF PPO IN ‘DELICIOUS’ APPLES DURING CONTROLLED ATMOSPHERE STORAGE. Journal of Food Biochemistry, 15: 185–199. doi: 10.1111/j.1745-4514.1991.tb00154.x

Author Information

  1. 1

    Department of Food Science and Technology Oregon State University Corvallis, OR 97331

  2. 2

    Department of Food Science and Technology New York State Agricultural Experiment Station Cornell University Geneva, NY 14456

  3. 3

    Department of Pomology Cornell University Ithaca, NY 14850

*1 Address correspondence to: Dr. Diane M. Barrett, Dept. of Food Science & Technology, Wiegand Hall, Oregon State University, Corvallis, OR 97331.

Publication History

  1. Issue published online: 23 FEB 2007
  2. Article first published online: 23 FEB 2007
  3. Received for Publication September 4, 1990 Accepted for Publication April 8, 1990

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ABSTRACT

Polyphenol oxidase (PPO) activity in 4, 000 × g, 100, 000 × g, 200 × g and soluble fractions of ‘Delicious’ apples was monitored during 28 weeks of controlled atmosphere storage. PPO activity in 4, 000 × g and 100, 000 × g fractions decreased, while that in soluble and 200 × g fractions increased. These shifts in subcellular location occurred sooner in apples which were stored under high CO2 conditions (2.5–6% O2, 8–12% CO2) than those under normal CA conditions (2% O2, 3% CO2). Polyacrylamide gel electrophoresis of 4, 000 × g fractions isolated from ‘Delicious’ apples at harvest showed 2 bands with PPO activity, while stored samples all had 3 bands. Isoenzyme patterns were found to vary in different subcellular fractions.

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