An experimental investigation was carried out to evaluate the quality of virgin olive oils obtained by intervarietal cross-breeding program. Twelve extra virgin olive oil cultivars were characterized by their chemical composition. The experimental data showed that new releases were characterized by an appreciable α-tocopherol (ranging from 60.09 to 153.97 mg/kg) and by a high content of total volatile compounds especially of the trans-2-hexenal. Simple phenols such as hydroxytyrosol, tyrosol, vanillic acid, p-coumaric acid, ferulic acid and vanillin were identified and quantified by high-performance liquid chromatography in most of the oils. The flavonoids compounds (apigenin and luteolin) were also found. The results indicated that a considerable oil composition variability can be achieved genetically, by breeding.