ANTIMICROBIAL ACTIVITY OF VARIOUS PARTS OF CINNAMOMUM CASSIA EXTRACTED WITH DIFFERENT EXTRACTION METHODS

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Abstract

ABSTRACT

The aim of this study is to compare the antibacterial activities of various parts (barks, buds and leaves) of Cinnamomum cassia extracted with ethanol and supercritical fluid extraction (SFE). The results obtained from the disk diffusion method and minimum inhibitory concentration (MIC) demonstrated that the SFE extracts of buds displayed significant antibacterial activity against the drug resistance pathogens, with MIC range between 0.3 and 0.7 mg/mL. Following the spectrophotometer analysis, (E)-cinnamaldehyde, O-methoxycinnamaldehyde, coumarin and eucalyptol were evidenced to be the major antimicrobial components in the SFE extracts. The antimicrobial mechanism was also investigated. The results suggested that the active components possess bactericide activity and interact on the cell membranes. In conclusion, the SFE extracts of buds have the potential value as an antibiotic substitute based on the in vitro antimicrobial assay. In addition, this study also provides a best SFE extraction condition to obtain high purity and yield of bioactive substances from C. cassia.

PRACTICAL APPLICATIONS

Cinnamon has been used as a preservative and for medicinal purposes for a long time in the East. The present study investigates the antibacterial activity of the alcohol and supercritical fluid extracts prepared from Cinnamomum cassia against the clinical antibiotic-resistant pathogens. Following the bioassays including minimum inhibitory concentration determination, synergistic effect, time-killing curve, scanning electron microscopy observation and spectrophotometric analysis, the results identify the chemical constituents and provide a method to isolate the highly purified active components from the extracts. The chemical components (E)-cinnamaldehyde, O-methoxycinnamaldehyde, coumarin and eucalyptol of C. cassia extracts exhibited antibacterial activities against different clinical isolates. These extracts of C. cassia could be developed as a natural disinfectant for treatment with nosocomial pathogens and could control antibiotic-resistant bacterium.

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