The aim of this study is to elucidate the anti-inflammatory effects of Carpesium abrotanoides. The chloroform- and ethyl acetate-fraction of C. abrotanoides had the potent inhibitory effect on nitrite production from lipopolysaccharide (LPS)-induced macrophage cells. The inhibition of NO production by C. abrotanoides extract-fractions might be both partially a result of chemical scavenge of NO and the suppression of iNOS transcription level in LPS-induced macrophage cells. In addition, C. abrotanoides extract-fractions inhibited significantly the biosynthesis of PGE2, and COX-2, which regulates the synthesis of PGE2, was attenuated partially by the treatment of C. abrotanoides extract-fractions in LPS-induced macrophage cells. Also, the hexane-, ethyl acetate- and aqueous-fraction of C. abrotanoides suppressed IL-1β level significantly almost to the basal (approximately 77.5–92.8% inhibition) in concentration-dependant manner. It is concluded that C. abrotanoides extract-fractions may be useful as a functional food material and an alternative medicine for the relief and retardation of immunological inflammatory responses.