Groundnut oil (GNO) was modified to contain equal proportions of omega-6/omega-3 fatty acids by blending with linseed oil. Blended oil (BLE) was subjected to interesterification reaction using immobilized lipase to prepare transesterified oil (TRA). A structured lipid (STR) was also prepared by acidolysis reaction using GNO and omega-3 fatty acid concentrates from linseed oil. These oils were then fed to rats for a period of 60 days. A reduction in low density lipoprotein cholesterol level by 26 and 23% and a reduction in serum triglyceride concentration by 18.9 and 17.6% were observed in rats given TRA and STR, respectively, as compared with rats fed with GNO. Rats fed with BLE and interesterified oils showed incorporation of α-linolenic acid and an increase in eicosapentaenoic acid and docosahexaenoic acid in serum and liver lipids. These modified oils could be used for enriching Indian diets with omega-3 fatty acids.