Analyses of lipid profile and fatty acid compositions of muscle and viscera of two commercially cultured abalone, namely Haliotis discus hannai Ino and Haliotis diversicolor Reeve, have been carried out. The total lipid levels of the viscera of both abalone were much higher than those of muscle. Phospholipids were the predominant lipid components in muscle, whereas triacylglycerol in viscera. The major fatty acids identified in muscle and viscera were C16:0, C18:0, C20:4n–6, C20:5n–3 and C22:5n–3. Polyunsaturated fatty acids (PUFAs) accounted for over 40% of total fatty acids, and C20:4n–6 and C20:5n–3 were the most abundant PUFAs. Moreover, different fatty acid compositions were found between the muscle and viscera. Compared with muscle, viscera contained higher percentage of 4,8,12-trimethyltridecanoic acid and C18-PUFA, but lower percentage of C22-PUFA and dimethyl acetals. This study would increase awareness of the nutritional value of these two abalone and also contribute some important information to abalone aquaculture.