ANTIOXIDANT POTENTIAL AND OXIDATIVE DNA DAMAGE PREVENTIVE ACTIVITY OF CHRYSANTHEMUM INDICUM EXTRACTS

Authors

  • TRISHNA DEBNATH,

    1. College of Biomedical & Health Science, Department of Life Science, Konkuk University, 322 Danwol-dong, Chungju-shi, Chungcheongbuk-do, 380-701, South Korea
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  • HAI LAN JIN,

    1. College of Biomedical & Health Science, Department of Life Science, Konkuk University, 322 Danwol-dong, Chungju-shi, Chungcheongbuk-do, 380-701, South Korea
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  • MD ABUL HASNAT,

    1. College of Biomedical & Health Science, Department of Life Science, Konkuk University, 322 Danwol-dong, Chungju-shi, Chungcheongbuk-do, 380-701, South Korea
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  • YUNSUK KIM,

    1. College of Biomedical & Health Science, Department of Life Science, Konkuk University, 322 Danwol-dong, Chungju-shi, Chungcheongbuk-do, 380-701, South Korea
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  • NADIRA BINTE SAMAD,

    1. College of Biomedical & Health Science, Department of Life Science, Konkuk University, 322 Danwol-dong, Chungju-shi, Chungcheongbuk-do, 380-701, South Korea
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  • PYO-JAM PARK,

    1. College of Biomedical & Health Science, Department of Life Science, Konkuk University, 322 Danwol-dong, Chungju-shi, Chungcheongbuk-do, 380-701, South Korea
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  • BEONG OU LIM

    Corresponding author
    1. College of Biomedical & Health Science, Department of Life Science, Konkuk University, 322 Danwol-dong, Chungju-shi, Chungcheongbuk-do, 380-701, South Korea
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TEL: 82-43-840-3570; FAX: 82-43-856-3572; EMAIL: beongou@kku.ac.kr

Abstract

ABSTRACT

The objective of this study was to characterize the antioxidant properties of the extracts from Chrysanthemum indicum flower (CIF). The water and ethanol extracts were prepared from CIF and its antioxidant activity was investigated in vitro by different methods including 1,1-diphenyl-2-picrylhydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, hydroxyl and superoxide radicals and nitrite scavenging, reducing (Fe3+ to Fe2+) power, linoleic acid oxidation inhibition as well as free radical-induced DNA damage prevention activity. The cytotoxic effect of the ethanol extract was determined on chang normal liver cell. The ethanol-based extract prevented free radical-induced DNA damage and did not show any cytotoxicity. The antioxidant activity was highly correlated with the observed phenolic and flavonoid contents. The present study suggests that the extracts of CIF could be considered as a potent source of natural antioxidants.

PRACTICAL APPLICATIONS

Chrysanthemum indicum Linné has been used as a traditional medicine for various immune-related diseases as well as in mixed spices and alcoholic beverages in Korea, China and Japan. The tea prepared from chrysanthemum flower could prevent sore throat and promote the reduction of fever. Recently, Chrysanthemum indicum flower (CIF) extracts has been reported to have therapeutic potential in a variety of immune-related cutaneous diseases and lowering blood pressure activity. In this context, we studied antioxidant activities and DNA damage preventive activity of CIF. The results suggest that the extract may have potential as a new source of natural antioxidant.

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