The work was carried out at Minzu University of China, Beijing, China.
Antioxidant Activities In Vitro of Ethanol Extract and Fractions from Mushroom, Lenzites Betulina †
Article first published online: 15 JUN 2012
© 2012 Wiley Periodicals, Inc
Journal of Food Biochemistry
Volume 37, Issue 6, pages 687–693, December 2013
How to Cite
Liu, K., Wang, J.-L., Gong, W.-Z., Xiao, X. and Wang, Q. (2013), Antioxidant Activities In Vitro of Ethanol Extract and Fractions from Mushroom, Lenzites Betulina . Journal of Food Biochemistry, 37: 687–693. doi: 10.1111/j.1745-4514.2012.00666.x
- Issue published online: 4 DEC 2013
- Article first published online: 15 JUN 2012
- Manuscript Accepted: 15 FEB 2012
- National Basic Research Program of China. Grant Number: 973 Program, 2009CB522300
- Fund of State Key Laboratory of Phytochemistry and Plant Resources in West China. Grant Number: 09708211Z1
- Program of State Ethnic Affairs Commission. Grant Number: 09ZY09
- “985” Project. Grant Number: MUC985
- “111” Project. Grant Number: B08044
- Fundamental Research Funds for the Central Universities. Grant Number: 2009kyqnyjs22, 0910KYZY46
The antioxidant potential of different fractions of Lenzites betulina was investigated. Fraction 2 showed the highest antioxidant activity in the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radical-scavenging assays. Three natural p-terphenyls were identified in this fraction. The structure of a new natural compound was established as 2-phenyl-[1H-2-benzopyran][4,3-e][p]benzoquinone in addition to two other compounds, 2,5-diphenyl-3,6-dimethoxy-p-benzoquinone and 2-phenyl-3-methoxy-[1H-2-benzopyran][4,3-e][p]benzoquinone that have been previously reported in L. betulina. The antioxidant activities of these p-terphenyls were evaluated by DPPH and OH radical-scavenging assays. 2-phenyl-3-methoxy-[1H-2-benzopyran][4,3-e][p]benzoquinone was the most active in the OH radical-scavenging test (IC 50 = 37.69 μg/mL) and showed little inhibition in the DPPH assay. The chemical components of L. betulina therefore have some antioxidant capacities, and this species could be used as a potential source of new natural antioxidants.
Lenzites betulina has been used as a kind of medicinal fungus in China. In order to find its potential application, the antioxidant potential of L. betulina was investigated. It was found that the total ethanol extracts and the fraction 2 of L. betulina showed strong antioxidative capacity in the 2, 2-diphenyl-1-picrylhydrazyl and hydroxyl (OH) radical-scavenging assays. The OH radical-scavenging activity of the fraction 2 (IC 50 = 19.42 ± 0.4 μg/mL) was similar to that of ascorbic acid (IC 50 = 20.55 ± 0.2 μg/mL). Meanwhile, three natural p-terphenyl (a new natural compound and two known compounds) were identified in this fraction. Compound 2 showed the most activity in the OH radical-scavenging test (IC 50 = 37.69 μg/mL), so this species could be used as a potential source of new natural antioxidants.