A highly species-specific polymerase chain reaction (PCR) assay was developed for the authentic identification of goat. A product of 436 bp was amplified using newly designed primers against mitochondrial D-loop region. The possibility of cross-amplification was ruled out by considering as many as 25 other animal species. Suitability of the developed goat species-specific PCR assay was confirmed for in raw, cooked (60, 80 and 100C for 30 min) and micro-oven-processed meat samples (n = 20 each). A sensitivity of 0.1% was established for detection of adulteration and limit of detection of goat DNA was 0.1 pg. This investigation presents a novel PCR assay with its newly designed primers that could be used for the authentic identification of goat species.