ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF AILANTHUS ALTISSIMA SWINGLE LEAVE EXTRACT TO REDUCE FOODBORNE PATHOGENS AND SPOILING BACTERIA
Article first published online: 29 JUN 2009
© 2009, Wiley Periodicals, Inc.
Journal of Food Safety
Volume 29, Issue 4, pages 499–510, November 2009
How to Cite
RAHMAN, A., KIM, E. L. and KANG, S. C. (2009), ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF AILANTHUS ALTISSIMA SWINGLE LEAVE EXTRACT TO REDUCE FOODBORNE PATHOGENS AND SPOILING BACTERIA. Journal of Food Safety, 29: 499–510. doi: 10.1111/j.1745-4565.2009.00172.x
- Issue published online: 26 OCT 2009
- Article first published online: 29 JUN 2009
- Accepted for Publication April 19, 2008
The antibacterial and antioxidant potentials of methanolic extracts of Ailanthus altissima Swingle leaves were evaluated. Antibacterial activity was tested in vitro by agar disk diffusion method against 11 (six gram-positive and five gram-negative) foodborne bacteria. The methanol extract and its different polar subfractions inhibited significantly the growth of all six gram-positive bacteria: Listeria monocytogenes (ATCC 19116, ATCC 19118 and ATCC 19166), Staphylococcus aureus (ATCC 6538 and KCTC 1916) and Bacillus subtilis ATCC 6633 and two gram-negative bacteria: Pseudomonas aeruginosa KCTC 2004 and Escherichia coli ATCC 8739. The zones of inhibition of methanol extract and its derived different polar subfractions against the tested bacteria were found in the 12.1–23.2 mm range and the minimum inhibitory concentration values were recorded between 62.5 and 500 µg/mL. Antioxidant activity was evaluated by using 1,1-diphenyl-2-picrylhydrazyl assay. The free radical scavenging activity of ethyl acetate (EtOAc) fraction was superior to all other fractions (IC50 = 16.45 µg/mL) and was higher than synthetic antioxidant butylated hydroxyanisole (IC50 = 18.27 µg/mL). Furthermore, the amount of total phenolic compounds was determined and its content in EtOAc fraction (12.25%) was the highest compared with other extract or fractions.
The natural products derived from Ailanthus altissima Swingle may contribute to the development of new antimicrobial agents with potential applications in food industries as natural preservatives or flavoring agents to control foodborne pathogens. They can be used as growth inhibitors of Listeria monocytogenes, Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa, as well as some important foodborne pathogens and spoiling bacteria. The main reason for their suitability is their natural origin, which consumers find comforting and is beneficial for the environment, and the very low risk that pathogens will develop resistance to the mixture of components that make up the extracts with their apparent diversity of antibacterial mechanisms. These beneficial characteristics could increase food safety and shelf life.