The aim was to describe the inactivation kinetics of Escherichia coli O157:H7 suspended in apple juice after pulsed electric fields (PEF) and a subsequent storage under refrigeration. Escherichia coli O157:H7 showed a great PEF resistance in apple juice, when survivors were evaluated immediately after PEF. However, PEF-treated cells exhibited a great sensitivity to a subsequent holding in apple juice for 3 days. For instance, although a PEF treatment of 80 pulses at 35.0 kV/cm inactivated less than 0.5 log10 cell cycles, the maintenance of the samples up to 3 days at 4C caused an inactivation of 5.0 log10 cycles. An equation based on the Weibullian-like distribution accurately described the kinetics of cell inactivation.