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ABSTRACT

This study was carried out to determine the effect of infusing food grade organic acids on the shelf life of fresh beef kept at refrigeration temperature of 5C. Citric, lactic, acetic and tartaric acids were infused individually in concentrations of 0.5, 0.75 and 1.00% into fresh beef slices. Infusion was carried out under pulled of 29.5 in. Hg. for 20 min. Samples were dropped dried and stored at 5C for 28 days. pH, water activity, total plate count (TPC), thiobarbaturic acid values, Hunter color values and instrumental texture were determined. The pH value of the treated samples dropped from the initial pH of 5.30 (untreated) to 4.20–4.47 and the TPC values were lower than 107 colony-forming units/g on day 16, 20 and 28 in samples treated with 0.5, 0.75 and 1.00% acids, respectively. The instrumental texture of the treated samples was harder upon treatment, which decreased gradually during storage period. The maximum shelf life of treated beef was extended to 12–24 days and citric acid in concentration of 1.00% gave the best effect.

PRACTICAL APPLICATIONS

In this study four acids were infused individually in concentrations of 0.5, 0.75 and 1.00% into fresh beef slices to determine the effect of infusing technology on its shelf life at refrigerated temperature of 5C. Samples were dried and stored at 5C for 28 days. pH, water activity, total plate count, thiobarbaturic acid values, Hunter color values and instrumental texture were determined. The treatments with higher acids concentrations minimized microorganism's growth and reduced the development of lipid oxidation.