To determine if spinach grown near cattle feedyards can become contaminated with microorganisms, fresh spinach bundles were set upright in polyvinyl chloride pipes within a feedyard. Bundles were located at 0, 20 and 50 yards from the cattle loadout area (dust generation). Control samples were in a Rubbermaid box (Rubbermaid, Huntersville, NC) to prevent environmental contamination. All samples were tested for generic Escherichia coli, E. coli O157 and Salmonella after 6, 12 and 24 h of feedyard exposure. Generic E. coli levels in exposed spinach placed at 0 yards increased by 2.5, 2.6 and 2.7 logs after 6, 12 and 24 h, respectively. These increases were significant when compared with control samples (P = 0.0456). At 20 and 50 yards, there was an increase in generic E. coli, with significantly less E. coli on spinach placed at farther distances. After 24 h, 50-yard samples contained 12.96 logs less Salmonella than 0-yard samples.