In Alaska, walleye pollock (WP [Theragra chalcogramma]) and Pacific cod (PC [Gadus macrocephalus]) are harvested in large amounts. There are also large biomasses of arrow-tooth flounder (ATF [Atheresthes stomias]) and giant grenadier (GG [Albatrossia pectoralis]) available in Alaskan waters; however, these fish are underutilized. The objective of this study was to determine lipid content, lipid class distribution and fatty acid (FA) profiles of fillets from these four fish species. Fat content and percent triacylglycerides (TAG) in ATF was significantly higher than in other species, and percent phospholipids (PL) was lower (P < 0.05). PC had the highest levels of omega-3 (ω-3) FA, whereas ATF had the lowest. The ratio of ω-3/omega-6 (ω-6) FA was significantly higher in ATF than in other species. Percent polyunsaturated and saturated FA's were equivalent in ATF; however, other species showed at least twice as much polyunsaturated FA as saturated FA. This study provided comparisons of the lipid characteristics in the two most abundant white fish harvested in Alaska and two other abundant but underutilized species.