Association of polymorphisms in low-density lipoprotein receptor-related protein 5 gene with bone mineral density in postmenopausal Chinese women1

Authors

  • Zhen-lin ZHANG,

    Corresponding author
    1. Center for Preventing and Treating Osteoporosis, Osteoporosis Research Unit, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
      2 Correspondence to Dr Zhen-lin ZHANG. Phn/Fax 86-21-6408-1474. E-mail ZZL2002@medmail.com.cn
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  • Yue-juan QIN,

    1. Center for Preventing and Treating Osteoporosis, Osteoporosis Research Unit, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
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  • Jin-wei HE,

    1. Center for Preventing and Treating Osteoporosis, Osteoporosis Research Unit, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
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  • Qi-ren HUANG,

    1. Center for Preventing and Treating Osteoporosis, Osteoporosis Research Unit, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
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  • Miao LI,

    1. Center for Preventing and Treating Osteoporosis, Osteoporosis Research Unit, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
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  • Yun-qiu HU,

    1. Center for Preventing and Treating Osteoporosis, Osteoporosis Research Unit, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
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  • Yu-juan LIU

    1. Center for Preventing and Treating Osteoporosis, Osteoporosis Research Unit, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
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  • 1

    Project supported by the grant from the Natural Science Foundation of Shanghai (No 03ZR 14056).

2 Correspondence to Dr Zhen-lin ZHANG. Phn/Fax 86-21-6408-1474. E-mail ZZL2002@medmail.com.cn

Abstract

Aim: To investigate the possible association of Q89R, N740N and A1330V polymorphisms in low-density lipoprotein receptor-related protein 5 (LRP5) gene with bone mineral density (BMD) in postmenopausal Chinese women.

Methods: Q89R, N740N and A1330V genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 647 unrelated healthy postmenopausal Han Chinese women aged 43–76 years in Shanghai. BMD at lumbar spine 1–4 and the left proximal femur including the femoral neck, trochanter and Ward's triangle were measured by dual-energy X-ray absorptionmetry in all subjects.

Results: The distribution of the Q89R, N740N and A1330V genotypes in this population was as follows: QQ 80.5%, QR 18.7%, and RR 0.8%; TT 66.9%, TC31.1%, and CC2.0%;AA 68.0%, AV 29.7%, and W 2.3%. The frequencies of the Q89R, N740N and A1330V genotypes and alleles did not deviate from the Hardy-Weinberg equilibrium. We found that the Q89R and A1330V polymorphisms were in linkage disequilibrium in our population (χ2=13.50, P<0.01). Both before and after adjusting for age, years since menopause, height, and weight, the Q89R or N740N genotypes were significantly associated with BMD at the femoral neck (P<0.05). Subjects with the Q89R QQ genotype or the N740N TT genotype had a significantly higher BMD at the femoral neck, compared with those with the QR/RR or TC/CC genotypes, respectively. No significant association was found between A1330V polymorphism and BMD at any site.

Conclusion: Our findings suggest that the LRP5 gene is a candidate for the genetic determination of BMD in postmenopausal Chinese women.

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