Project supported by a grant from the “863” program of China (No 2003AA2Z347C).
Role of rat liver cytochrome P450 3A and 2D in metabolism of imrecoxib1
Article first published online: 1 MAR 2006
Acta Pharmacologica Sinica
Volume 27, Issue 3, pages 372–380, March 2006
How to Cite
XU, H.-y., XIE, Z.-y., ZHANG, P., SUN, J., CHU, F.-m., GUO, Z.-r. and ZHONG, D.-f. (2006), Role of rat liver cytochrome P450 3A and 2D in metabolism of imrecoxib. Acta Pharmacologica Sinica, 27: 372–380. doi: 10.1111/j.1745-7254.2006.00265.x
- Issue published online: 1 MAR 2006
- Article first published online: 1 MAR 2006
- Received 2005-07-30 Accepted 2005-09-20
- cytochrome P450;
- liver microsomes
Aim: To investigate the in vitro metabolism of imrecoxib in rat liver microsomes and to identify the cytochrome P450 (CYP) forms involved in its metabolism. Methods: Liver microsomes of Wistar rats were prepared using an ultracentrifuge. The in vitro metabolism of imrecoxib was studied by incubation with rat liver microsomes. To characterize the CYP forms involved in the 4′-methyl hydroxylation of imrecoxib, the effects of typical CYP inducers (such as dexamethasone, isoniazid and β-naphthoflavone) and of CYP inhibitors (such as ketoconazole, quinine, α-naphthoflavone, methylpyrazole, and cimetidine) on the formation rate of 4′-hydroxymethyl imrecoxib were investigated. Results: Imrecoxib was metabolized to 3 metabolites by rat liver microsomes: 4′-hydroxymethyl imrecoxib (M4), 4′-hydroxymethyl-5-hydoxyl imrecoxib (M3), and 4′-hydroxymethyl-5-car-bonyl imrecoxib (M5). Over the imrecoxib concentration range studied (5–600 μmol/L), the rate of 4′-methyl hydroxylation conformed to monophasic Michaelis-Menten kinetics. Dexamethasone significantly induced the formation of M4. Ketoconazole markedly lowered the metabolic rate of imrecoxib in a concentration-dependent manner. Moreover, a significant inhibitory effect of quinine on the formation of M4 was observed in microsomes obtained from control rats, isoniazid-induced rats, and β-naphthoflavone-induced rats. In contrast, α-naphthoflavone, cimetidine, and methylpyrazole had no inhibitory effects on this metabolic pathway. Conclusion: Imrecoxib is metabolized via 4′-methyl hydroxylation in rat liver microsomes. The reaction is mainly catalyzed by CYP 3 A. CYP 2D also played a role in control rats, in isoniazid-induced rats and in β-naphthoflavone-induced rats.