Project supported by grants from the Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences (No 231766).
Anti-inflammatory effect of amurensin H on asthma-like reaction induced by allergen in sensitized mice1
Article first published online: 5 JUN 2006
Acta Pharmacologica Sinica
Volume 27, Issue 6, pages 735–740, June 2006
How to Cite
LI, Y.-t., YAO, C.-s., BAI, J.-y., LIN, M. and CHENG, G.-f. (2006), Anti-inflammatory effect of amurensin H on asthma-like reaction induced by allergen in sensitized mice. Acta Pharmacologica Sinica, 27: 735–740. doi: 10.1111/j.1745-7254.2006.00330.x
- Issue published online: 5 JUN 2006
- Article first published online: 5 JUN 2006
- Received 2005-12-14 Accepted 2006-01-27
- amurensin H;
- tumor necrosis factor-α;
- Th2 cells;
Aim: To explore the anti-inflammatory effects of amurensin H on asthma-like reaction induced by allergen in sensitized mice. Methods: BALB/c mice were sensitized by ovalbumin (OVA, ip) on d 0 and d 14 and challenged with 1 % OVA on d 18 to 22. Mice developed airway eosinophilia, mucus hypersecretion, and elevation in cytokine levels. Mice were administered amurensin H orally at the doses of 49, 70, or 100 mg/kg once every day from d 15 to the last day. Bronchoalveolar lavage fluid (BALF) were collected at 24 h and 48 h after the last OVA challenge. Levels of tumor necrosis factor-α (TNF-α), interleukin 4 (IL-4), interleukin 5 (IL-5), and interleukin 13 (IL-13) in BALF were measured using ELISA method. Differential cell counts of macrophages, lymphocytes, neutrophils and eosinophils were performed in 200 cells per slide (one slide per animal). Lung tissue sections of 6-um thickness were stained with Mayer's hematoxylin and eosin for assessment of cell infiltration, mucus production, and tissue damage. Results: Oral administration of amurensin H significantly inhibited OVA-induced increases in total cell counts, eosinophil counts, and TNF-α, IL-4, IL-5 and IL-13 levels in BALF. In addition, amuresin H dramatically decreased OVA-induced lung tissue damage and mucus production. Conclusion: Amurensin H may have therapeutic potential for the treatment of allergic airway inflammation.