The interaction of small molecules with phospholipid membranes studied by 1H NOESY NMR under magic-angle spinning1

Authors

  • Holger A SCHEIDT,

    1. Junior Research Group “Structural Biology of Membrane Proteins”, Institute of Biochemistry/Biotechnology, Martin Luther University Halle-Wittenberg, D-06120 Halle, Germany
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  • Daniel HUSTER

    Corresponding author
    1. Institute of Medical Physics and Biophysics, Univeristy of Leipzig, D-04107 Leipzig, Germany
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  • 1

    The study was supported by the “Exzellenz-netzwerk Biowissenschaften” funded by the federal state of Sachsen-Anhalt.

Correspondence to Dr Daniel HUSTER. Phn 49-345-552-4942. Fax 49-345-552-7013. E-mail daniel.huster@biochemtech.unihalle.de

Abstract

The interaction of small molecules with lipid membranes and the exact knowledge of their binding site and bilayer distribution is of great pharmacological importance and represents an active field of current biophysical research. Over the last decade, a highly resolved 1H solid-state NMR method has been developed that allows measuring localization and distribution of small molecules in membranes. The classical solution 1H NMR NOESY technique is applied to lipid membrane samples under magic-angle spinning (MAS) and NOESY cross-relaxation rates are determined quantitatively. These rates are proportional to the contact probability between molecular segments and therefore an ideal tool to study intermolecular interactions in membranes. Here, we review recent 1H MAS NOESY applications that were carried out to study lateral lipid organization in mixed membranes and the interaction of membranes with water, ethanol, small aromatic compounds, peptides, fluorescence labels, and lipophilic nucleosides.

Abbreviations
DMPC

1,2-dimyristoyl-sn-glycero-3-phosphocholine

DLPC

1,2-dilauroyl-sn-glycero-3-phosphocholine

DOPC

1,2-di-oleoyl-sn-glycero-3-phosphocholine

HOESY

heteronuclear Overhauser enhancement spectros copy

MAS

magic-angle spinning

MeDOPE

monomethyl-1,2-dioleoyl-sn-glycero-3-phosphoethanolamine

NBD

(7-nitro-2-1,3-benzoxadiazol-4-yl)amino

NOE

nuclear Overhauser effect

NOESY

nuclear Overhauser enhancement spectroscopy

Phe

phenylalanine

POPC

1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine

SDPE

1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine

SDPC

1-stearoyl-2- docosahexaenoyl -sn-glycero-3-phosphocholine

SDPS

1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphoserine

SOPC

1-stearoyl-2- oleoyl -sn-glycero-3-phosphocholine

Trp

tryptophane

Ancillary