This research was supported by a research fund from Shanghai Green Valley Holding.
Reversal effect of Ganoderma lucidum polysaccharide on multidrug resistance in K562/ADM cell line1
Version of Record online: 27 APR 2008
© 2008 CPS and SIMM
Acta Pharmacologica Sinica
Volume 29, Issue 5, pages 620–627, May 2008
How to Cite
LI, W.-d., ZHANG, B.-d., WEI, R., LIU, J.-h. and LIN, Z.-b. (2008), Reversal effect of Ganoderma lucidum polysaccharide on multidrug resistance in K562/ADM cell line. Acta Pharmacologica Sinica, 29: 620–627. doi: 10.1111/j.1745-7254.2008.00776.x
- Issue online: 27 APR 2008
- Version of Record online: 27 APR 2008
- Received 2007-10-18Accepted 2007-12-18
- multidrug resistance;
- Ganoderma lucidum polysaccharides;
- multidrug resistance-1;
- multidrug resistance-associated protein-1
Aim: To investigate the reversal effect of Ganoderma lucidum polysaccharides (Gl-PS) on multidrug resistance (MDR) in the adriamycin (ADM)-resistant leukemic cell line K562/ADM. Methods: Cytotoxicity was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method; the ADM concentration in cells was determined by flow cytometry and confocal laser scanning microscopy techniques; the expression of P-glycoprotein was assayed by flow cytometry; and the mRNA expression levels of MDR-1 and MDR-associated protein (MRP)1 were determined by RT-PCR Results:Gl-PS reversed MDR in K562/ADM cells. Gl-PS obviously reversed the resistance of K562/ADM to doxorubicin. The reversing factors of Gl-PS at 10 and 20 mg/L were 6.46 and 6.80, respectively. MDR-1 and MRP 1 transcription were downregulated by 10 and 50 mg/L Gl-PS. Conclusion:Gl-PS can reverse the MDR by downregulating the expression of MDR-1 and MRP1 in K562/ADM cells.