Expression of Basic Fibroblast Growth Factor Results in the Decrease of Myostatin mRNA in Murine C2C12 Myoblasts

Authors

  • Hua-Zhong LIU,

    1. State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100094, China
    2. Modern Biochemistry Center, Guangdong Ocean University, Zhanjiang 524088, China
    Search for more papers by this author
  • Qing LI,

    1. State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100094, China
    Search for more papers by this author
  • Xing-Yuan YANG,

    1. State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100094, China
    Search for more papers by this author
  • Lin LIU,

    1. State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100094, China
    Search for more papers by this author
  • Lei LIU,

    1. State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100094, China
    Search for more papers by this author
  • Xiao-Rong AN,

    Corresponding author
    1. State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100094, China
    Search for more papers by this author
  • Yong-Fu CHEN

    1. State Key Laboratory for Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100094, China
    Search for more papers by this author

  • This work was supported by a grant from the National High Technology Research and Development Program of China (2002AA206311)

*Corresponding author: Tel, 86-10-62733355; E-mail, xra@cau.edu.cn

Abstract

Abstract During the development and regeneration of skeletal muscle, many growth factors, such as basic fibroblast growth factor (bFGF, FGF-2) and myostatin, have been shown to play regulating roles. bFGF contributes to promote proliferation and to inhibit differentiation of skeletal muscle, whereas myostatin plays a series of contrasting roles. In order to elucidate whether the expression of bFGF has any relationship with the expression of myostatin in skeletal muscle cells, we constructed a eukaryotic expression vector for the expression of exogenous bFGF in murine C2C12 myoblasts. Quantitative RT-PCR assays indicated that with the increase of the expression of exogenous bFGF gene, the expression of endogenous myostatin gene was suppressed at mRNA level and protein level.

Edited by Hui-Ming JIN

Ancillary