Fourier Transform Infrared Spectroscopic Analysis of Protein Secondary Structures


  • This work was supported by the grants from the National Natural Science Foundation of China (No. 20745001 and 20525519), the National Basic Research Program of China (2007CB914304), and the Educational Foundation of Fudan University (EYH1615002)

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Infrared spectroscopy is one of the oldest and well established experimental techniques for the analysis of secondary structure of polypeptides and proteins. It is convenient, non-destructive, requires less sample preparation, and can be used under a wide variety of conditions. This review introduces the recent developments in Fourier transform infrared (FTIR) spectroscopy technique and its applications to protein structural studies. The experimental skills, data analysis, and correlations between the FTIR spectroscopic bands and protein secondary structure components are discussed. The applications of FTIR to the secondary structure analysis, conformational changes, structural dynamics and stability studies of proteins are also discussed.