Comparative proteomics analysis of light responses in cryptochrome1-304 and Columbia wild-type 4 of Arabidopsis thaliana


  • This work was supported by the grants from the “985” Program of China (No. 200501), the Department of Education of Hunan Province Fund (No. 04C328), and the National Natural Science Foundation of China (No. 30770200)

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The blue light photoreceptor mutant cryptochrome1-304 (cry1-304) and Columbia wild-type 4 (col-4) of Arabidopsis thaliana were grown under white light and blue light, and in the dark. To study the difference in protein expression levels between cry1-304 and col-4, a proteomic approach was applied based on 2-D gel electrophoresis. Twenty-one different protein spots were identified by matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry. The expression of four genes corresponding to four protein spots was analyzed by semiquantitative reverse transcription-polymerase chain reaction. We applied analytical procedures to study cry1-304 and col-4, and found that the differentially expressed proteins formed six clusters reflecting co-regulation. This assessment was consistent with the known physiological responses of plants to light.