Department of Biochemistry, Zhejiang Medical College, Hangzhou 310053, China
1745-7270/asset/ABBS_left.gif?v=1&s=d7c03567db265ca2593d3081e6c1f5eb7fdd879e)
1745-7270/asset/ABBS_right.gif?v=1&s=d6d18fc5d1585069e311beddd193156526022c20)
Interaction between angiogenin and fibulin 1: evidence and implication
Article first published online: 15 MAY 2008
DOI: 10.1111/j.1745-7270.2008.00420.x
© 2008 Institute of Biochemistry and Cell Biology, SIBS, CAS
Additional Information
How to Cite
Zhang, H., Gao, X., Weng, C. and Xu, Z. (2008), Interaction between angiogenin and fibulin 1: evidence and implication. Acta Biochimica et Biophysica Sinica, 40: 375–380. doi: 10.1111/j.1745-7270.2008.00420.x
Publication History
- Issue published online: 15 MAY 2008
- Article first published online: 15 MAY 2008
- Received: January 20, 2008 Accepted: March 17, 2008
- Abstract
- References
- Cited By
Keywords:
- angiogenin;
- fibulin 1;
- protein interaction;
- FRET;
- angiogenesis
Angiogenin is an angiogenic factor involved in tumorigenesis. However, the mechanism of angiogenin's action remains elusive. In the present study, we identified fibulin 1, an extracellular matrix and plasma glycoprotein, as an angiogenin-interacting molecule by yeast two-hybrid screening. This interaction was further confirmed by two different approaches. First, fibulin 1 was co-immunoprecipitated with angiogenin by anti-angiogenin monoclonal antibody in vitro, suggesting angiogenin binds with fibulin 1 directly. Then fluorescence resonance energy transfer analysis showed that fibulin 1 interacted with angiogenin in COS-7 cells, showing that the binding could occur in a cellular context. As fibulin 1 plays an important role in cell proliferation, migration, adhesion, and stabilizes new-forming blood vessel wall, the interaction between fibulin 1 and angiogenin might underline one possible mechanism of angiogenin in angiogenesis and/or tumorigenesis.