These authors contributed equally to this work
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Expression of Cbl-interacting protein of 85 kDa in MPTP mouse model of Parkinson's disease and 1-methyl-4-phenyl-pyridinium ion-treated dopaminergic SH-SY5Y cells
Article first published online: 28 JUN 2008
DOI: 10.1111/j.1745-7270.2008.00423.x
© 2008 Institute of Biochemistry and Cell Biology, SIBS, CAS
Additional Information
How to Cite
Bian, M., Yu, M., Yang, S., Gao, H., Huang, Y., Deng, C., Gao, Y., Sun, F. and Huang, F. (2008), Expression of Cbl-interacting protein of 85 kDa in MPTP mouse model of Parkinson's disease and 1-methyl-4-phenyl-pyridinium ion-treated dopaminergic SH-SY5Y cells. Acta Biochimica et Biophysica Sinica, 40: 505–512. doi: 10.1111/j.1745-7270.2008.00423.x
Publication History
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- Received: February 15, 2008 Accepted: April 9, 2008
- Abstract
- References
- Cited By
Keywords:
- CIN85;
- shRNA;
- SH-SY5Y cell;
- Parkinson's disease
The newly discovered Cbl-interacting protein of 85 kDa (CIN85) is involved in many cellular processes, but its functions in the brain and in neurodegenerative diseases remain unclear. In this paper, we investigated the distribution of CIN85 protein in different regions of adult mouse brain using Western blot analysis and immunohistochemistry, and found that CIN85 was ubiquitously expressed in mouse brain. In the striatum and substantia nigra, two regions most deeply affected in Parkinson's disease, the level of CIN85 protein was relatively high. In the MPTP mouse model of Parkinson's disease, the expression of CIN85 in the striatum and substantia nigra was complicated. But in 1-methyl-4-phenyl-pyridinium ion-treated human dopaminergic SH-SY5Y cells, the expression of CIN85 increased dramatically. Knocking down of CIN85 by short hairpin RNA reduced SH-SY5Y cell death. Therefore, CIN85 might play different roles in the dopaminergic cell line and in the nigrostriatum of mouse brain under neurotoxin challenge.