Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191

  1. Haoyue Wang1,†,
  2. Ruilin Sun3,4,†,
  3. Guoxiang Liu3,4,
  4. Minghui Yao3,4,
  5. Jian Fei2,*,
  6. Hebai Shen1,*

Article first published online: 12 AUG 2008

DOI: 10.1111/j.1745-7270.2008.00450.x

Issue

Acta Biochimica et Biophysica Sinica

Acta Biochimica et Biophysica Sinica

Volume 40, Issue 8, pages 704–710, August 2008

Additional Information

How to Cite

Wang, H., Sun, R., Liu, G., Yao, M., Fei, J. and Shen, H. (2008), Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191. Acta Biochimica et Biophysica Sinica, 40: 704–710. doi: 10.1111/j.1745-7270.2008.00450.x

Author Information

  1. 1

    Department of Chemistry, Life and Environmental Science College, Shanghai Normal University, Shanghai 200234, China

  2. 2

    School of Life Science and Technology, Tongji University, Shanghai 200092, China

  3. 3

    Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

  4. 4

    Graduate School of Chinese Academy of Sciences, Beijing 100049, China

  1. †

    These authors contributed equally to this work

* *Corresponding authors: Jian Fei: Tel, 86-21-65982429; Fax, 86-21-65982429; E-mail, jfei@mail.tongji.edu.en Hebai Shen: Tel, 86-21-64321800; Fax, 86-21-54242640; E-mail, shenhb@shnu.edu.cn

Publication History

  1. Issue published online: 12 AUG 2008
  2. Article first published online: 12 AUG 2008
  3. Received: March 29, 2008 Accepted: May 30, 2008

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Keywords:

  • zinc finger protein 191;
  • DNA-binding protein;
  • microsatellite

Studies on the DNA-binding properties of transcription factors are important in searching for the downstream genes regulated by these factors. In the present study, we report on the DNA-binding property of a Cy s2His2-type transcription factor, zinc finger protein 191 (Zfpl91), which has been newly found to play a significant role in mice. By constructing a fusion protein containingthe DNA-binding domain of Zfpl91, we characterized target DNA by determining the protein's binding specificity and dependence on zinc. The data showed that the DNA-binding domain of Zfpl91 can specifically bind to the TCAT repeat motif and that there is a cooperative effect amongthe target DNA's multiple binding sites. Furthermore, the binding reaction is dependent on zinc. This work provides a foundation for further studies on the role of Zfpl91 in gene regulation and development.

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