SarA influences the sporulation and secondary metabolism in Streptomyces coelicolor M145

  1. Xijun Ou1,
  2. Bo Zhang1,
  3. Lin Zhang1,
  4. Kai Dong1,
  5. Chun Liu1,
  6. Guoping Zhao1,2,3,*,
  7. Xiaoming Ding1,*

Article first published online: 17 OCT 2008

DOI: 10.1111/j.1745-7270.2008.00466.x

Issue

Acta Biochimica et Biophysica Sinica

Acta Biochimica et Biophysica Sinica

Volume 40, Issue 10, pages 877–882, October 2008

Additional Information

How to Cite

Ou, X., Zhang, B., Zhang, L., Dong, K., Liu, C., Zhao, G. and Ding, X. (2008), SarA influences the sporulation and secondary metabolism in Streptomyces coelicolor M145. Acta Biochimica et Biophysica Sinica, 40: 877–882. doi: 10.1111/j.1745-7270.2008.00466.x

Author Information

  1. 1

    State Key Laboratory of Genetic Engineering, Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China

  2. 2

    Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Centre at Shanghai, Shanghai 201203, China

  3. 3

    Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China

* *Corresponding authors: Guoping Zhao: Tel, 86-21-50801919; Fax, 86-21-50801922; E-mail, gpzhao@sibs.ac.cn Xiaoming Ding: Tel, 86-21-65643616; Fax, 86-21-65650149; E-mail, xmding74@fudan.edu.cn

Publication History

  1. Issue published online: 17 OCT 2008
  2. Article first published online: 17 OCT 2008
  3. Received: June 26, 2008 Accepted: August 6, 2008

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Keywords:

  • Streptomyces coelicolor;
  • sporulation;
  • antibiotic production;
  • sarA

The filamentous bacteria Streptomyces exhibit a complex life cycle involving morphological differentiation and secondary metabolism. A putative membrane protein gene sarA (sco4069), sporulation and antibiotic production related gene A, was partially characterized in Streptomyces coelicolor M145. The gene product had no characterized functional domains and was highly conserved in Streptomyces. Compared with the wild-type M145, the sarA mutant accelerated sporulation and dramatically decreased the production of actinorhodin and undecylprodigiosin. Reverse transcription-polymerase chain reaction analysis showed that SarA influenced antibiotic production by controlling the abundance of actII-orf4 and redZ messenger RNA.

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