Transgenic breeding of anti-Bombyx mori L. nuclear polyhedrosis virus silkworm Bombyx mori

  1. Huijuan Yang1,
  2. Wei Fan1,
  3. Hao Wei1,
  4. Jinwei Zhang2,
  5. Zhonghua Zhou1,
  6. Jianying Li1,
  7. Jianrong Lin3,
  8. Nong Ding2,
  9. Boxiong Zhong1,*

Article first published online: 17 OCT 2008

DOI: 10.1111/j.1745-7270.2008.00471.x

Issue

Acta Biochimica et Biophysica Sinica

Acta Biochimica et Biophysica Sinica

Volume 40, Issue 10, pages 873–876, October 2008

Additional Information

How to Cite

Yang, H., Fan, W., Wei, H., Zhang, J., Zhou, Z., Li, J., Lin, J., Ding, N. and Zhong, B. (2008), Transgenic breeding of anti-Bombyx mori L. nuclear polyhedrosis virus silkworm Bombyx mori. Acta Biochimica et Biophysica Sinica, 40: 873–876. doi: 10.1111/j.1745-7270.2008.00471.x

Author Information

  1. 1

    College of Animal Sciences, Zhejiang University, Hangzhou 310029, China

  2. 2

    Huzhou Academy of Agriculture Sciences, Huzhou 313000, China

  3. 3

    College of Animal Sciences, South China Agricultural University, Guangzhou 510642, China

* *Corresponding author: Tel/Fax, 86-571-86971302; E-mail, bxzhong@zju.edu.cn

Publication History

  1. Issue published online: 17 OCT 2008
  2. Article first published online: 17 OCT 2008
  3. Received: July 07, 2008 Accepted: August 27, 2008

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Keywords:

  • silkworm;
  • transgenic;
  • piggyBac;
  • BmNPV;
  • antivirotic

Silkworm strains resistant to Bombyx mori L. nuclear polyhedrosis virus were obtained through transgenic experiments. piggyBac transposon with an A3 promoterwere randomly inserted into the silkworm, driving the enhanced green fluorescent protein (EGFP) reporter gene into the silkworm genome. Polymerase chain reaction results verified the insertion of the extraneous EGFP gene, and fluorescence microscopy showed that the EGFP was expressed in the midgut tissue. The morbidity ratio of the nuclear polyhedrosis decreased from 90% in the original silkworm strain to 66.7% in the transgenic silkworm strain. Compared with the resistance to the Bombyx mori L. nuclear polyhedrosis virus in the Qiufeng strain, which is commonly used in the production, there was an increase of 33 centesimal points in the transgenic silkworms. The antivirotic character in the ChunhuaxQiuyue strain, which was bred from a different transgenic family, was about 10 centesimal points higher than that in the QiufengxBaiyu, another crossbreed used in production. Our results indicated a good application value of the transposon-inserted mutation in the breeding of anti-BmNPV silkworm strain.

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