Expression and characterization of rice putative PAUSED gene

  1. Chengguo Yao1,3,†,
  2. Liangfa Ge2,3,†,
  3. Wei Li1,3,
  4. Botao Zhao1,3,
  5. Chaoqun Li4,
  6. Kangcheng Ruan1,3,*,
  7. Hongxuan Lin2,3,
  8. Youxin Jin1,3,*

Article first published online: 17 OCT 2008

DOI: 10.1111/j.1745-7270.2008.00472.x

Issue

Acta Biochimica et Biophysica Sinica

Acta Biochimica et Biophysica Sinica

Volume 40, Issue 10, pages 893–900, October 2008

Additional Information

How to Cite

Yao, C., Ge, L., Li, W., Zhao, B., Li, C., Ruan, K., Lin, H. and Jin, Y. (2008), Expression and characterization of rice putative PAUSED gene. Acta Biochimica et Biophysica Sinica, 40: 893–900. doi: 10.1111/j.1745-7270.2008.00472.x

Author Information

  1. 1

    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai 200031, China

  2. 2

    State Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai 200032, China

  3. 3

    Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

  4. 4

    Department of Infectious Diseases, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China

  1. †

    These authors contributed equally to this work

* *Corresponding author: Youxin Jin: Tel, 86-21-54921222; Fax, 86-21-54921011; Email, yxjin@sibs.ac.cn Kangcheng Ruan: Fax, 86-21-54921011; Email, kcruan@sibs.ac.cn

Publication History

  1. Issue published online: 17 OCT 2008
  2. Article first published online: 17 OCT 2008
  3. Received: May 08, 2008 Accepted: August 31, 2008

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Keywords:

  • PAUSED;
  • tRNA export;
  • development defects;
  • rice

In Arabidopsis, PAUSED (PSD) encodes the ortholog of los1p/exportin-t, which mediates the nuclear export of transfer RNA (tRNA) in yeast and mammals. However, in monocot plants such as rice, knowledge of the corresponding ortholog is limited, and its effects on growth development and productivity remain unknown. In this study, we verified a rice transfer-DNAinsertional mutant psd line and analyzed its phenotypes; the mutant displayed severe morphological defects including retarded development and low fertility compared with wild-type rice. Examining intronless tRNA-Tyr and intron-containing pre-tRNA-Ala expression levels in cytoplasmic and nuclear fraction with Northern blot analysis between wild-type and mutant leaf tissue suggested that rice PSD might be involved in tRNA export from the nucleus to the cytoplasm. Additionally, reverse transcription-polymerase chain reaction analysis revealed that PSD transcript was expressed throughout normal rice plant development, and subcellular localization assays showed that rice PSD protein was present in both the nucleus and cytoplasm. In summary, our data implied that the putative PSD gene might be indispensable for normal rice development and its function might be the same as that of Arabidopsis PSD.

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