These authors contributed equally to this work
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Specific expression of short-interfering RNA driven by human telomerase reverse transcriptase promoter in tumor cells
Article first published online: 1 DEC 2008
DOI: 10.1111/j.1745-7270.2008.00475.x
© 2008 Institute of Biochemistry and Cell Biology, SIBS, CAS
Additional Information
How to Cite
Luan, X., Guo, L., Yang, Z., Liu, M., Li, X. and Tang, H. (2008), Specific expression of short-interfering RNA driven by human telomerase reverse transcriptase promoter in tumor cells. Acta Biochimica et Biophysica Sinica, 40: 928–933. doi: 10.1111/j.1745-7270.2008.00475.x
Publication History
- Issue published online: 1 DEC 2008
- Article first published online: 1 DEC 2008
- Received: August 19, 2008 Accepted: September 24, 2008
- Abstract
- References
- Cited By
Keywords:
- RNAi;
- short-interfering RNA;
- telomerase;
- hTERT promoter;
- gene therapy
RNA interference (RNAi) has been shown to be an effective method for inhibiting the expression of a given gene in human cells by targeting with short duplex RNA (short-interfering RNA or siRNA). However, more and more studies suggest that non-specific effects can be induced by siRNAs, such as off-target inhibition, activation of interferon response, and saturation of cellular silencing machinery. It has been known that more than 90% of human tumors exhibit telomerase activity. Consequently, telomerase is believed to be a broadspectrum molecular marker of malignancies. In the present study we attempt to develop a tumor-specific RNAi system using the human telomerase reverse transcriptase promoter. This system may provide a basis for RNAi therapy.