Proliferative Effect of Calcitonin Gene-related Peptide is Induced by at least Five Proteins as Identified by Proteome Profiling

Authors

  • Franka Pluder,

    1. Institute of Biochemistry, Faculty of Bioscience, Pharmacy and Psychology, University of Leipzig, Brüderstrasse 34, D-04103 Leipzig, Germany
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  • Andrea Sinz,

    1. Biotechnological-Biomedical Center, Faculty of Chemistry and Mineralogy, University of Leipzig, Linnéstrasse 3, D-04103 Leipzig, Germany
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  • Annette G. Beck-Sickinger

    Corresponding author
    1. Institute of Biochemistry, Faculty of Bioscience, Pharmacy and Psychology, University of Leipzig, Brüderstrasse 34, D-04103 Leipzig, Germany
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* Annette G. Beck-Sickinger,
beck-sickinger@uni-leipzig.de

Abstract

Calcitonin gene-related peptide is a 37 amino acid neuropeptide. Although calcitonin gene-related peptide activates a G-protein-coupled receptor, recent evidence suggests that calcitonin gene-related peptide induces more complex signaling cascades including the activation of MAP kinases [Eur J Pharmacol; 389:125–130 (2000), Neuropeptides; 34:229–233 (2000)]. However, the molecular mechanisms of this activation still remain to be elucidated. For the first time we applied a proteomics approach in order to identify molecular targets of calcitonin gene-related peptide downstream signaling in the neuroblastoma cell line SK-N-MC and identified proteins that changed either their expression, location, or their post-translational modifications in a time-dependent manner after calcitonin gene-related peptide stimulation.

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