Anna Lamberti and Nicola M. Martucci equally contributed to this work.
Interaction Between the Antibiotic Tetracycline and the Elongation Factor 1α from the Archaeon Sulfolobus solfataricus
Article first published online: 16 JUN 2011
© 2011 John Wiley & Sons A/S
Chemical Biology & Drug Design
Volume 78, Issue 2, pages 260–268, August 2011
How to Cite
Lamberti, A., Martucci, N. M., Ruggiero, I., Arcari, P. and Masullo, M. (2011), Interaction Between the Antibiotic Tetracycline and the Elongation Factor 1α from the Archaeon Sulfolobus solfataricus. Chemical Biology & Drug Design, 78: 260–268. doi: 10.1111/j.1747-0285.2011.01142.x
- Issue published online: 13 JUL 2011
- Article first published online: 16 JUN 2011
- Accepted manuscript online: 20 MAY 2011 12:42PM EST
- Received 23 July 2010, revised 1 February 2011 and accepted for publication 23 April 2011
- elongation factor 1α;
- protein synthesis;
- Sulfolobus solfataricus;
The interaction between tetracycline and the archaeal elongation factor 1α from Sulfolobus solfataricus was investigated. The effects produced by this eubacterial antibiotic indicated that this interaction involved the G-domain of the elongation factor 1α from S. solfataricus, although also the M-domain was required. In fact, in the presence of the antibiotic, an increase in the fluorescence quantum yield of the aromatic region was observed for elongation factor 1α from S. solfataricus and its truncated form lacking the C-terminal domain, but not for that lacking also the M-domain. The increase in quantum yield was restored when the G-domain of elongation factor 1α from S. solfataricus was fused to the M and the C-domains of the eubacterial analogue elongation factor Tu. Tetracycline inhibits protein synthesis catalysed by elongation factor 1α from S. solfataricus; this is accompanied by an increase in the GDP/GTP exchange rate and a slight inhibition of the intrinsic GTPase, suggesting that a main effect of the antibiotic was exerted on the GTP-bound form of the enzyme. Furthermore, the mixed inhibition observed for GTPase confirmed that the interaction, besides the G-domain, involved also other region(s) of elongation factor 1α from S. solfataricus. These results can be useful for studying potential side effects arising from the interaction between tetracycline and eukaryotic elongation factors.