Isolation of a novel root-determined hypernodulation mutant rdh1 of Lotus japonicus
Article first published online: 1 APR 2008
© 2008 Japanese Society of Soil Science and Plant Nutrition
Soil Science & Plant Nutrition
Volume 54, Issue 2, pages 259–263, April 2008
How to Cite
ISHIKAWA, K., YOKOTA, K., LI, Y. Y., WANG, Y., LIU, C.-T., SUZUKI, S., AONO, T. and OYAIZU, H. (2008), Isolation of a novel root-determined hypernodulation mutant rdh1 of Lotus japonicus. Soil Science & Plant Nutrition, 54: 259–263. doi: 10.1111/j.1747-0765.2007.00240.x
- Issue published online: 1 APR 2008
- Article first published online: 1 APR 2008
- Received 18 October 2007.Accepted for publication 11 November 2007.
- Lotus japonicus;
A mutant line that develops an excess number of small nodules was found in Lotus japonicus Miyakojima MG20 during a screening for mutants defective in nodule development and nitrogen fixation. Genetic analysis revealed that the phenotype is inherited in a monogenic, recessive manner. The gene's locus was mapped on chromosome 1 between 53.7 and 61.4 cM. This mutant formed 5–10-fold more nodules than the wild-type plant, and a grafting experiment revealed that the root regulated the hypernodulation. Except for the nodulation's phenotype no other differences were found between the mutant and the wild-type plant with respect to growth and morphological characteristics. In the mapped locus for the mutant no nodulation genes were reported, and this fact strongly suggests that the gene's locus is a new one. The gene was named root-determined hypernodulation (rdh) 1.