Shoot-synthesized nodulation-restricting substances are present in the medium-polarity fraction of shoot extracts from wild-type soybean plants

Authors

  • Hiroko YAMAYA,

    1. United Graduate School of Agricultural Science
    Search for more papers by this author
    • Present address: Division of Plant Sciences, Plant–Microbe Interactions Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.

  • Yasuhiro ARIMA

    1. Department of Bioproduction, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan
    Search for more papers by this author

Y. ARIMA, Department of Bioproduction, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan. Email: AND46529@nifty.com

Abstract

Previously, we reported that shoot-synthesized nodulation-restricting substance(s) (SNRS) was present in extracts from shoots of a wild-type soybean, cv. Williams82. In the present paper, we report SNRS activity of fractions prepared from shoot extracts of Williams82. Activity of the ethanol-soluble medium-polarity fraction (MPF), which was prepared by solid-phase extraction of the shoot extract using an octadecyl-bonded silica (ODS) cartridge, was similar to that of the shoot extract. A high-polarity fraction of the extract was separated into subfractions, which were tested for SNRS activity. The anionic plus non-ionic fraction and the cationic plus amphoteric fraction showed no SNRS activity. Our findings indicate that SNRS is present in the MPF of an extract prepared from shoots of Williams82.

Ancillary