Evaluation of the antifungal activity of four solutions used as a final rinse in vitro

Authors

  • Zahed Mohammadi DMD, MSD,

    Corresponding author
    1. Department of Endodontics, Hamedan University of Medical Sciences, Hamedan, Iran
    2. Iranian Centre for Endodontic Research (ICER), Tehran, Iran
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  • Luciano Giardino MD, DDS,

    1. Department of Periodontology, Endodontology, Pharmacology and Microbiology, Dental School, University of Brescia, Brescia, Italy
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  • Flavio Palazzi MD, DDS

    1. Department of Odontostomatological and Maxillofacial Sciences, Federico II University of Naples, Naples, Italy
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Dr Zahed Mohammadi, Department of Endodontics, Hamedan University of Medical Sciences, Hamedan 65176-59114, Iran. Email: mohammadi_zahed@yahoo.com

Abstract

The aim of the study was to compare the antifungal activity of 1.3% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), MTAD and Tetraclean as a final rinse against Candida albicans in a human tooth model in vitro. Ninety extracted human maxillary central and lateral incisor teeth were randomly divided into four groups each with 20 teeth, a positive and a negative control each with five teeth. After preparing the root canals, teeth were inoculated with Candida albicans (ATCC 10261) and incubated for 72 h. Teeth were divided into four experimental groups according to the irrigation solution as follows: NaOCl, CHX, MTAD and Tetraclean. After culturing aliquots from the experimental teeth on Sabouraud 4% dextrose agar, colony-forming units were counted. The results showed that 1.3% NaOCl and 2% CHX were equally effective and significantly superior to MTAD and Tetraclean (P < 0.05). Furthermore, antifungal efficacy of Tetraclean was significantly superior to MTAD (P < 0.05).

Ancillary