These authors contributed equally to this work.
Pre- and post-treatment with cyclosporine A in a rat model of transient focal cerebral ischaemia with multimodal MRI screening
Article first published online: 7 AUG 2012
© 2012 The Authors. International Journal of Stroke © 2012 World Stroke Organization
International Journal of Stroke
Volume 8, Issue 8, pages 669–674, December 2013
How to Cite
Cho, T.-H., Aguettaz, P., Campuzano, O., Charriaut-Marlangue, C., Riou, A., Berthezène, Y., Nighoghossian, N., Ovize, M., Wiart, M. and Chauveau, F. (2013), Pre- and post-treatment with cyclosporine A in a rat model of transient focal cerebral ischaemia with multimodal MRI screening. International Journal of Stroke, 8: 669–674. doi: 10.1111/j.1747-4949.2012.00849.x
Conflict of interest: None declared.
- Issue published online: 25 NOV 2013
- Article first published online: 7 AUG 2012
- acute stroke therapy;
- focal cerebral ischaemia;
- MRI ;
Irreversible damage may occur at reperfusion after sustained cerebral ischaemia.
We investigated the value of cyclosporine A for reducing the infarct size in a model of transient middle cerebral artery occlusion.
Twenty-seven Sprague-Dawley rats sustained a middle cerebral artery occlusion of one-hour. Acute multimodal Magnetic Resonance Imaging (MRI) was used during occlusion to confirm the success of surgery and measure baseline lesion size. Animals were randomly treated by: (i) intracarotid cyclosporine A (10 mg/kg) 20 mins before middle cerebral artery occlusion (pretreatment group); (ii) intracarotid cyclosporine A (10 mg/kg) immediately after reperfusion (post-treatment group); and (iii) intracarotid saline immediately after reperfusion.
Histopathological measurements on day 1 showed a significant reduction of infarct size in the pretreatment group compared to the post-treatment (percentage values of ipsilateral hemispheres: 16 ± 5% vs. 29 ± 11%, P = 0·004) and saline groups (16 ± 5% vs. 42 ± 12%, P = 0·015). No significant difference was observed between the post-treatment and saline groups (P = 0·065). Behavioural examinations on day 1 showed no significant difference between groups. Immunohistochemistry showed a statistically significant reduction of microglial cell count in the pretreatment group compared to either saline or cyclosporine A post-treatment groups.
We conclude that intracarotid cyclosporine A is effective in reducing infarct size when given prior to ischaemia, but not when administered at reperfusion.