Botany School, Cambridge, England.
Osmotic Behaviour of the Epithelial Cells of Frog Skin
Version of Record online: 8 DEC 2008
© 1961 Scandinavian Physiological Society
Acta Physiologica Scandinavica
Volume 53, Issue 3-4, pages 348–365, November 1961
How to Cite
MacRobbie, E. A. C. and Ussing, H. H. (1961), Osmotic Behaviour of the Epithelial Cells of Frog Skin. Acta Physiologica Scandinavica, 53: 348–365. doi: 10.1111/j.1748-1716.1961.tb02293.x
- Issue online: 8 DEC 2008
- Version of Record online: 8 DEC 2008
- Received 24 June 1961
The osmotic behaviour of the frog skin epithelium has been investigated by microscopic measurements of volume under different experimental conditions. Simultaneous measurements of potential and short-circuit current were made. The effects of changes in tonicity of both bathing solutions and of ionic replacements (K+ for Na+ ***SO=4 for Cl-) were studied. Furthermore the active sodium transport was stimulated by short-circuiting and by application of antidiuretic hormone, and inhibited by low pH and by g-strophanthin.
The following conclusions could be drawn:
- 1) The outward facing boundary of the epithelium is permeable to Na+ and Cl-, but impermeable to K+ and SO=4.
- 2) The inward facing boundary is permeable to K+ and Cl- but practically impermeable to Na+ and SO=4.
- 3) The outward facing boundary is much less permeable to water than is the inward facing one.
- 4) Application of antidiuretic hormone to the inside bathing solution increases the water permeability of the outward facing boundary whereas the inward facing membrane is unaffected.
- 5) Inhibition of the active sodium transport by either g-strophanthin or by low pH in the inside bathing solution was accompanied by a pronounced decrease in the passive ion permeabilities.
- 6) The results strongly indicate that potassium is transported actively from the inside bathing solution into the epithelium.