Muscle type-specific response of PGC-1α and oxidative enzymes during voluntary wheel running in mouse skeletal muscle
Version of Record online: 20 OCT 2006
Volume 188, Issue 3-4, pages 217–223, November/December 2006
How to Cite
Ikeda, S., Kawamoto, H., Kasaoka, K., Hitomi, Y., Kizaki, T., Sankai, Y., Ohno, H., Haga, S. and Takemasa, T. (2006), Muscle type-specific response of PGC-1α and oxidative enzymes during voluntary wheel running in mouse skeletal muscle. Acta Physiologica, 188: 217–223. doi: 10.1111/j.1748-1716.2006.01623.x
- Issue online: 20 OCT 2006
- Version of Record online: 20 OCT 2006
- Received 9 June 2006, revision requested 7 July 2006, final revision received 7 August 2006, accepted 10 August 2006
- skeletal muscle;
- wheel running
Aim: It is generally accepted that endurance exercise increases the expression of peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), which governs the expression of oxidative metabolic enzymes. A previous report demonstrated that the regulation of mitochondrial protein expression in skeletal muscles in response to cold exposure depends on muscle fibre type. Cold exposure and endurance exercise are both metabolic challenges that require adjustments in mitochondrial energy metabolism, we hypothesized that the exercise-induced increase in oxidative enzymes and PGC-1α expression is higher in fast-type than in slow-type muscle.
Methods: Female ICR mice were individually housed in cages equipped with running wheel for 1, 2, 4, 6 or 8 weeks. The soleus, plantaris (PLA) and tibialis anterior (TA) muscles were then prepared from each mouse. The expression levels of PGC-1α, mitochondrial proteins and GLUT4 were evaluated by Western blotting.
Results: The expression level of PGC-1α was increased only in the PLA muscle. Furthermore, the expression levels of all mitochondrial proteins and GLUT4 in the PLA muscle were increased. In the TA muscle, although there was no increase in PGC-1α expression, the expression levels of mitochondrial proteins and GLUT4 were increased.
Conclusions: These results suggest that muscle type-specific responses occur during endurance exercise, and that the increase in PGC-1α expression is not the only factor that promotes oxidative capacity as a result of endurance exercise.