The expression patterns of Pax7 in satellite cells during overload-induced rat adult skeletal muscle hypertrophy
Article first published online: 19 SEP 2008
© 2008 The Authors. Journal compilation © 2008 Scandinavian Physiological Society
Volume 195, Issue 4, pages 459–469, April 2009
How to Cite
Ishido, M., Uda, M., Kasuga, N. and Masuhara, M. (2009), The expression patterns of Pax7 in satellite cells during overload-induced rat adult skeletal muscle hypertrophy. Acta Physiologica, 195: 459–469. doi: 10.1111/j.1748-1716.2008.01905.x
- Issue published online: 6 MAR 2009
- Article first published online: 19 SEP 2008
- Received 27 June 2008, revision requested 24 July 2008, revision received 2 September 2008, accepted 11 September 2008
- functional overload;
- satellite cell;
- skeletal muscle
Aim: Activated satellite cells (SCs) have the ability to reacquire a quiescent, undifferentiated state. Pax7 plays a crucial role in allowing activated SCs to undergo self-renewal. Because the increase in the SC population is induced during overload-induced skeletal muscle hypertrophy, it is possible that Pax7-regulated SC self-renewal is involved in the modulation of the SC population during the functional overload of skeletal muscles. However, the characteristics of the expression patterns of Pax7 in SCs during the functional overload of adult skeletal muscles are poorly understood.
Methods: Using immunohistochemical approaches, we examined the temporal and spatial expression patterns of Pax7 expressed in SCs during the functional overloading of rat skeletal muscles.
Results: The time course of Pax7 expression in SCs was similar to that of the expression of the differentiation regulatory factor myogenin during the early stage of functional overload. However, the percentage of SCs that expressed Pax7 was markedly higher than that of the SCs that expressed myogenin. Coexpression of Pax7 and myogenin was not detected in SCs. In addition, the expression of cyclin-dependent kinase inhibitor p21, which regulates cell cycle arrest and differentiation, was not detected in Pax7-positive SCs.
Conclusion: These results suggest that Pax7-regulated self-renewal of SCs may be induced during the early stage of functional overload and may contribute to modulating the SC population in hypertrophied muscles. Furthermore, it was suggested that the numbers of SCs which underwent self-renewal may be higher than that of SCs which were provided as the additional myonuclei for hypertrophying myofibres.