Orexin receptor subtype activation and locomotor behaviour in the rat
Article first published online: 4 NOV 2009
© 2009 The Authors. Journal compilation © 2009 Scandinavian Physiological Society
Special Issue: 16TH ACTA PHYSIOLOGICA INTERNATIONAL SYMPOSIUM ‘10 YEARS OF HYPOCRETINS/OREXINS-PHYSIOLOGY AND PATHOPHYSIOLOGY’
Volume 198, Issue 3, pages 313–324, March 2010
How to Cite
Samson, W. K., Bagley, S. L., Ferguson, A. V. and White, M. M. (2010), Orexin receptor subtype activation and locomotor behaviour in the rat. Acta Physiologica, 198: 313–324. doi: 10.1111/j.1748-1716.2009.02056.x
- Issue published online: 28 JAN 2010
- Article first published online: 4 NOV 2009
- Received 17 January 2009, revision requested 20 April 2009, final revision received 14 October 2009, accepted 28 October 2009
- locomotor behaviour;
- orexin receptors;
Aim: Orexin-producing neurones, located primarily in the perifornical region of the lateral hypothalamus, project to a wide spectrum of brain sites where they influence numerous behaviours as well as modulating the neuroendocrine and autonomic responses to stress. While some of the actions of orexin appear to be mediated via the type 1 receptor, some are not, including its action on the release of one stress hormone, prolactin. We describe here the ability of orexin to increase locomotor behaviours and identify the importance of both receptor subtypes in these actions.
Methods: Rats were tested for their behavioural responses to the central activation of both the type 1 (OX1R) and type 2 (OX2R) receptor (ICV orexin A), compared to OX2R activation using a relatively selective OX2R agonist in the absence or presence of an orexin receptor antagonist that possesses highest affinity for OX1R.
Results: Increases in locomotor activity were observed, effects which were expressed by not only orexin A, which binds to both the OX1R and the OX2R receptors, but also by the relatively selective OX2R agonist [(Ala11, Leu15)-orexin B]. Furthermore, the OX1R selective antagonist only partially blocked the action of orexin A on most locomotor behaviours and did not block the actions of [(Ala11, Leu15)-orexin B].
Conclusion: We conclude that orexin A exerts its effects on locomotor behaviour via both the OX1R and OX2R and that agonism or antagonism of only one of these receptors for therapeutic purposes (i.e. sleep disorders) would not provide selectivity in terms of associated behavioural side effects.