J. Eastwood’s current address is Department of Clinical Veterinary Sciences, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA
Pattern of Coombs’ test reactivity has diagnostic significance in dogs with immune-mediated haemolytic anaemia
Article first published online: 1 OCT 2008
© 2008 British Small Animal Veterinary Association
Journal of Small Animal Practice
Volume 49, Issue 10, pages 525–530, October 2008
How to Cite
Warman, S. M., Murray, J. K., Ridyard, A., Eastwood, J., Silva, S. and Day, M. J. (2008), Pattern of Coombs’ test reactivity has diagnostic significance in dogs with immune-mediated haemolytic anaemia. Journal of Small Animal Practice, 49: 525–530. doi: 10.1111/j.1748-5827.2008.00641.x
- Issue published online: 1 OCT 2008
- Article first published online: 1 OCT 2008
Objectives: To investigate the clinical significance of the pattern of Coombs’ test reactivity in dogs with immune-mediated haemolytic anaemia.
Methods: Sixty-five anaemic dogs with a positive Coombs’ test were included. Coombs’ testing was performed at 4 and 37°C with polyvalent canine Coombs’ reagent and antisera specific for each of canine immunoglobulin G, immunoglobulin M and complement factor C3. The impact of performing testing with only polyvalent antiserum at 37°C was assessed. Chi-squared tests were used to compare Coombs’ test reactivity in dogs with primary immune-mediated haemolytic anaemia (group A) and in dogs with concurrent/underlying disease (group B). Following Bonferroni correction, significance was set at P≤0·003.
Results: Eleven dogs would have been regarded as Coombs’ negative had they been tested with polyvalent antiserum at 37°C alone. Group A dogs were significantly more likely to be positive with polyvalent antiserum and/or anti-dog immunoglobulin G at 4 and/or 37°C (P≤0·001) and tended to be less likely to be positive with anti-dog immunoglobulin M at 4°C (P=0·040).
Clinical Significance: Testing of anaemic dogs with polyvalent Coombs’ reagent at 37°C was less sensitive than testing with monovalent reagents at 4 and 37°C. The pattern of Coombs’ test reactivity differed significantly between dogs with primary immune-mediated haemolytic anaemia and those with concurrent/underlying disease.