Evaluation of platelet activation in canine immune-mediated haemolytic anaemia
Article first published online: 1 JUN 2010
© 2010 British Small Animal Veterinary Association
Journal of Small Animal Practice
Volume 51, Issue 6, pages 296–304, June 2010
How to Cite
Ridyard, A. E., Shaw, D. J. and Milne, E. M. (2010), Evaluation of platelet activation in canine immune-mediated haemolytic anaemia. Journal of Small Animal Practice, 51: 296–304. doi: 10.1111/j.1748-5827.2010.00935.x
- Issue published online: 1 JUN 2010
- Article first published online: 1 JUN 2010
- Accepted: 20 January 2010
Objectives: To establish whether heightened platelet activation is a common feature of canine immune-mediated haemolytic anaemia, and to evaluate the hypothesis that platelet activation plays a role in the pathogenesis of thromboembolism.
Methods: Using whole-blood flow-cytometric analysis, the proportion of activated platelets and platelet-leucocyte aggregates in blood samples from 14 dogs with immune-mediated haemolytic anaemia and 14 healthy dogs was calculated. General linear models with binomial errors were used to compare groups. Results from the immune-mediated haemolytic anaemia-affected dogs were then correlated with established risk factors for thromboembolism in canine immune-mediated haemolytic anaemia, D-dimer concentration and antithrombin activity.
Results: There was a strong correlation between platelet activation and severe thrombocytopenia, with heightened platelet activation being observed predominantly in severely thrombocytopenic dogs.
Clinical Significance: Dogs with immune-mediated haemolytic anaemia, particularly those with concurrent severe thrombocytopenia, are likely to have heightened platelet activation, which may play a role in the pathogenesis of thromboembolism.