Presented in part at the ECVIM Congress, Seville, 8-10 September 2011.
Initial evaluation of canine urinary cystatin C as a marker of renal tubular function
Article first published online: 10 APR 2012
© 2012 British Small Animal Veterinary Association
Journal of Small Animal Practice
Volume 53, Issue 5, pages 254–259, May 2012
How to Cite
Monti, P., Benchekroun, G., Berlato, D. and Archer, J. (2012), Initial evaluation of canine urinary cystatin C as a marker of renal tubular function. Journal of Small Animal Practice, 53: 254–259. doi: 10.1111/j.1748-5827.2012.01198.x
- Issue published online: 26 APR 2012
- Article first published online: 10 APR 2012
- Accepted: 23 February 2012
Objectives: To evaluate the performance of a particle-enhanced turbidimetric assay for measuring canine urinary cystatin C and to investigate if the urinary cystatin C to creatinine ratio is higher in dogs with renal disease than in non-renal disease dogs.
Methods: Urinary cystatin C was measured by particle-enhanced turbidimetric assay using an avian antihuman cystatin C antibody and the performance of this assay was evaluated. Clinical relevance was tested in 46 dogs that were divided into three groups: healthy dogs (n=14), non-renal disease dogs (n=17) and dogs with renal disease (n=15).
Results: The assay was linear (R2=0·99) and precise (mean intra- and inter-assay coefficients of variation were 2·3 and 2·9%, respectively). The recovery was 111·5% and the limit of blank was 0·02 mg/L. Urinary cystatin C and urinary cystatin C to creatinine ratio differed significantly (P<0·001) between the three cohorts of dogs.
Clinical Significance: Measurement of cystatin C by particle-enhanced turbidimetric assay performed with high precision and linearity. This assay can be processed on automated clinical chemistry analysers making it widely available to commercial laboratories. Urinary cystatin C to creatinine ratio can differentiate dogs with renal disease from dogs without renal disease. These preliminary results suggest that urinary cystatin C to creatinine ratio is a promising marker for evaluating renal tubular function.