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Keywords:

  • Anopheles sinensis;
  • blood-feeding;
  • mosquito apolipophorin-III;
  • Korea

Abstract

Apolipophorin-III is known to play a role in transporting lipids in insects, and much attention has been paid to lepidopteran insects' apolipophorin. Thus, we were interested in examining the effects of blood-meal on the expression pattern of apolipophorin-III in mosquitoes. This led us to clone and partially characterize the full-length cDNA of apoLp-III (AnsiApoLp-III) from Anopheles sinensis. Analysis of AnsiApoLp-III cDNA shows that the 728-bp sequence has a 582-bp protein-coding region with 94 bp of putative 5′ untranslated region and 152 bp of 3′ untranslated region. The deduced amino acid sequence begins with a methionine codon at position 95 and extends to position 674, encompassing a polypeptide of 193 amino acids. AnsiApoLp-III has the highest identity (63%) to Culex quinquefasciatus apoLp-III. Temporal expression pattern analysis shows that although AnsiApoLp-III was expressed at all developmental stages, it was highly detected at egg and adult stages in the female mosquitoes. In addition, we found out that AnsiApoLp-III was induced in An. sinensis adult females after uptaking a blood-meal. Spatial expression patterns of AnsiApoLp-III shows that AnsiApoLp-III mRNA was strongly induced at day 1 and gradually decreased from day 1 to day 4 in the ovaries. Most interestingly, AnsiApoLp-III mRNA in the Malpighian tubule was strongly induced at day 1, decreased during days 1–3, and then became elevated again at day 4. These data suggest that blood-meal influences AnsiApoLp-III mRNA induction in ovaries and Malpighian tubules. It remains to further elucidate the biological roles of AnsiApoLp-III in these organs.