Isolation and analysis of natural compounds from silkworm pupae and effect of its extracts on alcohol detoxification
Article first published online: 17 JAN 2012
© 2012 The Authors. Entomological Research © 2012 The Entomological Society of Korea and Blackwell Publishing Asia Pty Ltd
Volume 42, Issue 1, pages 55–62, January 2012
How to Cite
KWON, M.-G., KIM, D.-S., LEE, J.-H., PARK, S.-W., CHOO, Y.-K., HAN, Y.-S., KIM, J.-S., HWANG, K.-A., KO, K. and KO, K. (2012), Isolation and analysis of natural compounds from silkworm pupae and effect of its extracts on alcohol detoxification. Entomological Research, 42: 55–62. doi: 10.1111/j.1748-5967.2011.00439.x
- Issue published online: 17 JAN 2012
- Article first published online: 17 JAN 2012
- Received 7 September 2011; accepted 7 November 2011.
- quercetin diglucoside;
- silkworm pupae;
- vitamin B2;
- α-linolenic acid
Silkworm pupae have much potential and many applications as a natural medicine to promote human health. However, their chemical components have not been fully characterized or understood. HPLC analysis was conducted to determine the content ratio (%) of individual amino acids in total protein of the pupae. It showed that glutamic acid (18.3%), histidine (14.6%) and alanine (10.2%) are the most common amino acids in silkworm pupae. Fatty acid composition of silkworm pupae oil was revealed by high-pressure liquid chromatography and gas chromatography – mass spectroscopy analyses. They contain a high ratio of essential fatty acids, [α-linolenic acid (ω-3 fatty acid]+ linoleic acid) (49.0%), and also contain non-essential fatty acids, oleic acid (19.9%), palmitoleic acid (2.5%), palmitic acid (19.7%), stearic acid (8.6%), and eicosapentaenoic acid (EPA) (0.3%). In addition, they also contain antioxidants, quercetin diglucoside and nutritionally important riboflavin (vitamin B2). This study suggests that silkworm pupae are a nutritionally valuable food product and are applicable as cosmetic components with essential amino acids, essential fatty acids, antioxidants and vitamins. The animal experiment showed that alcohol dehydrogenase (ADH) activity was significantly higher in the liver of mice orally administered with 0.5 mg/mL of silkworm extract and alcohol than with commercial Dawn808™ and alcohol, indicating that silkworm pupae extracts have alcohol detoxification activity.