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SKIN BIOPSY SAMPLING OF BELUGA WHALE CARCASSES: ASSESSMENT OF BIOPSY DARTING FACTORS FOR MINIMAL WOUNDING AND EFFECTIVE SAMPLE RETRIEVAL
Article first published online: 26 AUG 2006
Marine Mammal Science
Volume 11, Issue 2, pages 163–171, April 1995
How to Cite
PATENAUDE, N. J. and WHITE, B. N. (1995), SKIN BIOPSY SAMPLING OF BELUGA WHALE CARCASSES: ASSESSMENT OF BIOPSY DARTING FACTORS FOR MINIMAL WOUNDING AND EFFECTIVE SAMPLE RETRIEVAL. Marine Mammal Science, 11: 163–171. doi: 10.1111/j.1748-7692.1995.tb00515.x
- Issue published online: 26 AUG 2006
- Article first published online: 26 AUG 2006
- Received: 29 March 1993 Accepted: 30 August 1994
- biopsy darting;
- Delphinapterus leucas;
- DNA yield
Different combinations of biopsy tip lengths (20, 25 mm) and diameters (5, 6, 7 mm), crossbow draw weights (23, 45, 68 kg) and distances (1.5–15 m) were tested on fresh beluga carcasses to determine factors affecting the success of biopsy retrieval and the extent of wounding. Tips with smaller diameters and longer lengths were found to be more likely to retrieve a skin sample, while the draw weight of the crossbow had a significant effect on the severity of the wound. The samples obtained from all biopsy darts tested yielded sufficient amounts of DNA for genetic analysis (20–109 μg) with the highest yield coming from the germinativum spinosum layer. For beluga whales we recommend using a tip of 5 mm in diameter, 25 mm in length, and a draw weight of 23 kg at close range.