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Keywords:

  • Florida manatee;
  • Trichechs manatus latirostris;
  • interleukin 2 (IL-2);
  • cDNA

Abstract

Interleukin-2 (IL-2) production is essential for optimal mammalian immune response; therefore, a cDNA encoding IL-2 was isolated and characterized for the endangered Florida manatee, Trichechus manatus latirostris. The manatee IL-2 cDNA was cloned by reverse transcription/polymerase chain reaction (PCR), using primers derived from IL-2 regions conserved among various mammalian species. Manatee IL-2 cDNA consisted of 450 base pairs encoding a predicted product of 150 amino acid residues and displayed 87%, 82%, 79%, 78%, 75%, and 73% DNA sequence homology and 74%, 69%, 63%, 62%, 61%, and 57% amino acid sequence homology with human, pig, sheep, cow, rat, and mouse IL-2, respectively.