Current address: 723 Misty Lea, Houston, TX 77090.
ISOLATION AND CHARACTERIZATION OF A cDNA ENCODING INTERLEUKIN 2 FROM THE FLORIDA MANATEE, TRICHECHUS MANATUS LATIROSTRIS
Article first published online: 26 AUG 2006
Marine Mammal Science
Volume 12, Issue 1, pages 89–98, January 1996
How to Cite
Cashman, M. E., Ness, T. L., Roess, W. B., Bradley, W. G. and Reynolds, J. E. (1996), ISOLATION AND CHARACTERIZATION OF A cDNA ENCODING INTERLEUKIN 2 FROM THE FLORIDA MANATEE, TRICHECHUS MANATUS LATIROSTRIS. Marine Mammal Science, 12: 89–98. doi: 10.1111/j.1748-7692.1996.tb00306.x
- Issue published online: 26 AUG 2006
- Article first published online: 26 AUG 2006
- January 1995 Accepted: 17 April 1995
- Florida manatee;
- Trichechs manatus latirostris;
- interleukin 2 (IL-2);
Interleukin-2 (IL-2) production is essential for optimal mammalian immune response; therefore, a cDNA encoding IL-2 was isolated and characterized for the endangered Florida manatee, Trichechus manatus latirostris. The manatee IL-2 cDNA was cloned by reverse transcription/polymerase chain reaction (PCR), using primers derived from IL-2 regions conserved among various mammalian species. Manatee IL-2 cDNA consisted of 450 base pairs encoding a predicted product of 150 amino acid residues and displayed 87%, 82%, 79%, 78%, 75%, and 73% DNA sequence homology and 74%, 69%, 63%, 62%, 61%, and 57% amino acid sequence homology with human, pig, sheep, cow, rat, and mouse IL-2, respectively.