Appendix S1: The hierarchical regression analysis model used in this study.

Table S1: Multilevel analysis fitted (study area) and random (song) effect estimates and significance level for all the measured acoustic features.

Table S2: Results from multiple comparison analysis using Bonferroni correction for all five acoustic parameters from songs recorded in all study areas (GIB = Strait of Gibraltar; PROV = Provençal basin; COL = Columbretes archipelago; ALB = Alboran basin; AZO = Azores archipelago).

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