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From the data presented, it can be assumed that the increase in catecholamines and decrease in melatonin in the ovarian follicular fluid of IVF participants has physiological significance, particularly in the process of steroidogenesis. Furthermore, it is worthy of note that low SHBG levels in follicular fluid were associated with atretic oocytes. Therefore, NE, EP, ME, and SHBG titer, as well as E2 and LH measurements might usefully index prognoses for IVF.