Growth Hormone Receptors and Immunocompetent Cellsa

Authors

  • M. DARDENNE,

    Corresponding author
    1. Centre National de la Recherche Scientifique URA 1461, Hôpital Necker, 161 rue de Sèvres, 75743 Paris Cedex 15, France
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  • V. MELLO-COELHO,

    1. Centre National de la Recherche Scientifique URA 1461, Hôpital Necker, 161 rue de Sèvres, 75743 Paris Cedex 15, France
    2. Institut National de la Santé et de la Recherche Médicale Unité 344, Endocrinologie Moléculaire, 156 rue de Vaugirard, 75730 Paris Cedex 15, France
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  • M. C. GAGNERAULT,

    1. Centre National de la Recherche Scientifique URA 1461, Hôpital Necker, 161 rue de Sèvres, 75743 Paris Cedex 15, France
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  • M. C. POSTEL-VINAY

    1. Institut National de la Santé et de la Recherche Médicale Unité 344, Endocrinologie Moléculaire, 156 rue de Vaugirard, 75730 Paris Cedex 15, France
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    This work was supported by grants from INSERM, CNRS, Université Paris V. Valeria de Mello-Coelho is the recipient of a scholarship from the Brazilian Research Council (CNPq).

Additional correspondence information: Telephone: 00 33 01 44 49 53 92; Fax: 00 33 01 44 49 06 76; e-mail: dardenne@infobiogen.fr

Abstract

Abstract: Growth hormone plays a significant role in regulation of the humoral and cellular immune responses in physiological as well as pathological situations. This role is exerted via the existence of specific receptors on cells of the immune system. Using flow cytofluorometry and biotinylated bovine GH, we have recently analyzed the expression of GH receptors (GHRs) in murine lymphoid organs. GHRs are widely expressed in all murine hematopoietic tissues and in fetuses, newborns, and 3- and 7-week-old animals. In the bone marrow, all hematopoietic lineages express variable levels of GH receptors, whereas in the thymus, this expression is mainly seen in CD4, CD8, CD4+CD8+, and CD8+ subpopulations. At the periphery, 50% of splenocytes and peripheral blood lymphocytes and 20% of lymph node cells are GHR positive, with a wider receptor expression on B cells and macrophages (∼50%) than on T cells (∼20%), where the labeling is seen on both CD4+ and CD8+ cell subsets. Interestingly, the proportion of GHRs bearing CD4+ and CD8+ splenocytes is increased after T-cell activation with Con A or anti-CD3. Finally, all peripheral T cells expressing GHRs also express prolactin receptors. These data provide a molecular basis to study the factors controlling GHR expression and regulation of immune function.

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