Author to whom correspondence should be addressed at: USDA-ARS-ADRU, 337 Bustad Hall, Washington State University, Pullman, WA 99164-7030.
Babesia bovis Immunity: In Vitro and in Vivo Evidence for IL-10 Regulation of IFN-γ and iNOSa
Article first published online: 7 FEB 2006
Annals of the New York Academy of Sciences
How to Cite
GOFF, W. L., JOHNSON, W. C. and CLUFF, C. W. (1998), Babesia bovis Immunity: In Vitro and in Vivo Evidence for IL-10 Regulation of IFN-γ and iNOS. Annals of the New York Academy of Sciences, 849: 161–180. doi: 10.1111/j.1749-6632.1998.tb11046.x
This project was funded by USDA-ARS-ADRU Babesia project CWU-5348-34000-010-00D.
- Issue published online: 7 FEB 2006
- Article first published online: 7 FEB 2006
ABSTRACT: IL-10 has been shown to have profound immunoregulatory attributes and in the bovine appears to downregulate both Th1- and Th2-like responses. Using RT-PCR, we demonstrate IL-10 in vitro down-regulation of mRNA expression of iNOS, the cytokines involved in nitric oxide signal transduction initiation (IFN-γ and TNF-α), and other mononuclear phagocyte associate cytokines. In addition, using RT-PCR with peripheral blood leukocytes and spleen leukocytes, the Griess reaction, and a killing assay, we provide evidence for the importance of iNOS in a successful immune response to B. bovis infection and for high and persistent IL-10 mRNA expression when the immune response is unsuccessful. We also provide evidence that antibody developed early after an initial infection appears to lack protective attributes (neutralizing and opsonic). Together, the data suggests that IL-10 and IFN-γ are critical molecules involved in the response to this intraerythrocytic protozoan infection.